Sybr Green Gel Stain

When bound to nucleic acids the green fluorescent sybr safe stain has fluorescence excitation maxima at 280 and 502 nm and an emission maximum at 530 nm see figure.

Sybr green gel stain.

Lonza sybr green i nucleic acid gel stain for the detection of dsdna and oligonucleotides in agarose and polyacrylamide gels 446 50 781 50. Sybr safe dna gel stain is not classified as corrosive ignitable or reactive under the guidelines of the environmental protection agency epa table 1. Sybr green i stain has been shown to be much less mutagenic than ethidium bromide in ames tests. Sybr green i is also a very sensitive stain for oligonucleotides allowing for detection of as little as 1 2 ng of a synthetic 24 mer on a 5 polyacrylamide gel which is 50 100 times greater sensitivity than obtained with ethidium bromide.

The stain preferentially binds to double. Sybr green i sg is an asymmetrical cyanine dye used as a nucleic acid stain in molecular biology the sybr family of dyes is produced by molecular probes inc now owned by thermo fisher scientific sybr green i binds to dna the resulting dna dye complex best absorbs 497 nanometer blue light λ max 497 nm and emits green light λ max 520 nm. Because sybr green i has greater sensitivity for dsdna it is especially useful for assays where the presence of contaminating rna or ssdna might obscure results.